We have analysed a total of 100 patients with acute coronary syndrom (ACS) according to the severity and prognosis of their coronary artery disease by using a standard markers of acute myocardial infarction (AMI), as well as troponin and myoglobin test. We have made laboratory tests on the first day of acute symptoms. These results were compared to the standard laboratory tests (CK, CK-Mb and LDH). Our aim was to evaluate sensitivity and specificity of method. According to our results we concluded that troponin and myoglobin test became an important parameter in the diagnostic of ACS. Furthermore, troponin test have an imoprtant prognostic value in patients with unstablne angina and non-Q infarct, as well as in the patients with chest pain. Troponin test is a standard test in the judgment of myocardial necrosis and in the Western Europe it has become as the only parameter of diagnostic, prognosis and treatment.